Coding

Part:BBa_K108020:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-23)

Dam


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

Dam was cloned from E.coli K12 DH10B genome (reference 1).

References

1. NCBI.